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        "resumen" => "<p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">DNA alterations occurs frequently in sorne types of neoplasia such as retinoblastoma&#44; and lung&#44; breast and colorectal carcinoma&#44; We have found that loses of restriction fragments at the epidermal growth factor receptor gene &#40;EGEF-R&#41; are present in as much as 82&#37; of human brain tumours&#46; Amplifications of fragments are present at a much lower proportion reaching only 10&#37; of the samples examined&#46; Two fragments of 9<span class="elsevierStyleHsp" style=""></span>kb and 3&#46;6<span class="elsevierStyleHsp" style=""></span>kb are preferentially lost during tumorigenesis and the latter only is not present in 79&#37; of the cases in which normal and tumoral samples from the same individual were compared&#46; In all but one sample examined in which alterations are present&#44; the 3&#46;6<span class="elsevierStyleHsp" style=""></span>kb fragment was heterozygotic in normal tissues and was not present in the tumour&#46; Normal tissue surrounding the tumour was analyzed in a few examples and already showed a certain degree of alteration but normally not as much as the tumour itself&#46; This will suggest that an accumulation ofseveral DNA aberrations might be responsible for determining the tumour biological propertieso Restriction fragment length polymorphism &#40;RFLP&#41;&#44; irrelevant to tumorigenesis&#44; was found in about 22&#37; of the cases&#46;</p>"
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        "resumen" => "<p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">Alteraciones en las cadenas de DNA han sido detectadas en algunos tipos tumorales&#44; como retinoblastomas y carcinomas de pulm&#243;n&#44; mama y colon&#46; Por nuestra parte&#44; hemos observado que en el 82&#37; de los tumores cerebrales examinados existen p&#233;rdidas de fragmentos de DNA en el gen del receptor del factor de crecimiento epid&#233;rmico&#44; as&#237; como amplificaci&#243;n de otros fragmentos en el 10&#37; de los tumores&#46; Los fragmentos que m&#225;s frecuentemente faltan son dos&#44; uno de 9<span class="elsevierStyleHsp" style=""></span>kb &#40;kilobase&#41; y otro de 3&#44;6&#46; Este &#250;ltimo no se encontr&#243; en el 79&#37; de los casos en los que exist&#237;a en el tejido normal del mismo paciente&#46; En todos los casos excepto en uno&#44; este fragmento era heterozigoto en el tejido normal y faltaba en el tumoral&#46; El tejido aparentemente normal que rodea al tumor fue analizado en algunos casos&#44; observ&#225;ndose en &#233;l algunas anomal&#237;as&#44; aunque no tantas como en el propio tumor&#46; Esto sugiere que probablemente sean necesarias varias alteraciones del DNA para que aparezcan las propiedades tumorales&#46; Polimorfismo en la longitud de los fragmentos de restricci&#243;n fue observado en el 22&#37; de los casos&#46;</p>"
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                    0 => array:2 [
                      "titulo" => "A genetic model for colorectal tumorige nesis"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "E&#46;R&#46; Fearon"
                            1 => "B&#46; Vogelstein"
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                    ]
                  ]
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                    0 => array:1 [
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                        "tituloSerie" => "Cell"
                        "fecha" => "1990"
                        "volumen" => "61"
                        "paginaInicial" => "759"
                        "paginaFinal" => "767"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/2188735"
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                          ]
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                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "The human EGF receptor gene&#58; structure the 110<span class="elsevierStyleHsp" style=""></span>kb locus and identification sequences regulating its transcriptions"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
                            0 => "J&#46; Haley"
                            1 => "N&#46; Whittle"
                            2 => "P&#46; Bennett"
                            3 => "D&#46; Kinchington"
                            4 => "A&#46; Ullrich"
                            5 => "M&#46; Waterfleld"
                          ]
                        ]
                      ]
                    ]
                  ]
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                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "Oncongen Research"
                        "fecha" => "1987"
                        "volumen" => "1"
                        "paginaInicial" => "375"
                        "paginaFinal" => "396"
                      ]
                    ]
                  ]
                ]
              ]
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                      "titulo" => "Genetic of cancer predisposition"
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                            0 => "M&#46;F&#46; Hansen"
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                    ]
                  ]
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                        "paginaInicial" => "5518"
                        "paginaFinal" => "5527"
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                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/2889524"
                            "web" => "Medline"
                          ]
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                    ]
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                ]
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                      "titulo" => "Clonal genomic alterations in glioma malignancy stages"
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                            0 => "C&#46;D&#46; James"
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                            2 => "J&#46;P&#46; Dumanski"
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                            4 => "M&#46; Nordenskjold"
                            5 => "P&#46; Colllns"
                            6 => "W&#46;K&#46; Cavenee"
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                        ]
                      ]
                    ]
                  ]
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                    0 => array:1 [
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                        "volumen" => "48"
                        "paginaInicial" => "5546"
                        "paginaFinal" => "5551"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/2901288"
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                          ]
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                  ]
                ]
              ]
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                    0 => array:2 [
                      "titulo" => "Regulation of stability and synthesis of EGF receptor mRNAs encoding for intact and truncated receptor forms"
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                          "etal" => false
                          "autores" => array:4 [
                            0 => "P&#46; Kesavan"
                            1 => "P&#46; Das"
                            2 => "J&#46; Kern"
                            3 => "M&#46; Das"
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                        ]
                      ]
                    ]
                  ]
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                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Oncogene"
                        "fecha" => "1990"
                        "volumen" => "5"
                        "paginaInicial" => "483"
                        "paginaFinal" => "488"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/2326077"
                            "web" => "Medline"
                          ]
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                      ]
                    ]
                  ]
                ]
              ]
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                    0 => array:2 [
                      "titulo" => "Truncation of the human EGF receptor leads to differential lransforming potentials in primary avian fibroblasts and erythroblasts"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:7 [
                            0 => "K&#46; Khazaie"
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                            2 => "T&#46; Graf"
                            3 => "J&#46; Schelssinger"
                            4 => "A&#46; Ullrich"
                            5 => "H&#46; Beug"
                            6 => "B&#46; Vennstrom"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:4 [
                        "tituloSerie" => "EMBO J"
                        "fecha" => "1988"
                        "volumen" => "7"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/2468488"
                            "web" => "Medline"
                          ]
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                      ]
                    ]
                  ]
                ]
              ]
            ]
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                    0 => array:2 [
                      "titulo" => "Unique allelic restriction fragments of the human Ha-ras locus in leucocyte and tumour DNAs of cancer patients"
                      "autores" => array:1 [
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                          "etal" => false
                          "autores" => array:4 [
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                            2 => "M&#46; Colb"
                            3 => "D&#46;R&#46; Parkinson"
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                        ]
                      ]
                    ]
                  ]
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                      "Revista" => array:5 [
                        "tituloSerie" => "Nafure"
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                        "volumen" => "313"
                        "paginaInicial" => "369"
                        "paginaFinal" => "374"
                      ]
                    ]
                  ]
                ]
              ]
            ]
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                    0 => array:2 [
                      "titulo" => "HER2 cytoplasmic domain generates normal mitogenic and transforming signals in a chimeric receptor"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "J&#46; Lee"
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                            2 => "I&#46; Lax"
                            3 => "J&#46; Schlessinger"
                            4 => "A&#46; Ullrich"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:7 [
                        "tituloSerie" => "EMBO J"
                        "fecha" => "1989"
                        "volumen" => "8"
                        "paginaInicial" => "421"
                        "paginaFinal" => "427"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/2785915"
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                      "titulo" => "Amplification&#44; enhanced expressions and possible rearragement of EGF receptor gene in primary human brain tumours of glial origin"
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                            9 => "J&#46; Schelessinger"
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                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/2981413"
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              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Selective amplification on the cyloplasmic domain of the epidermal growth factor receptor gene in glioblastoma multiforme"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "I&#46;T&#46; Malden"
                            1 => "V&#46; Novac"
                            2 => "A&#46;H&#46; Kaye"
                            3 => "A&#46;W&#46; Buges"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Cancer Research"
                        "fecha" => "1988"
                        "volumen" => "48"
                        "paginaInicial" => "2711"
                        "paginaFinal" => "2714"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/2834047"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            10 => array:3 [
              "identificador" => "bib11"
              "etiqueta" => "11&#46;"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Genetic analysis of hyperproduction of epidermal growth factor receptors in human epidermoid carcinoma As431 cells"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "N&#46; Shimizu"
                            1 => "I&#46; Kondo"
                            2 => "M&#46;A&#46; Gamou"
                            3 => "A&#46; Behzadian"
                            4 => "Y&#46; Shimizu"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "Somatic Cell Mol&#46; Genet&#46;"
                        "fecha" => "1984"
                        "volumen" => "10"
                        "paginaInicial" => "45"
                        "paginaFinal" => "53"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            11 => array:3 [
              "identificador" => "bib12"
              "etiqueta" => "12&#46;"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Signal transduction by receptors with tyrosine kinase activity"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "A&#46; Ullrich"
                            1 => "J&#46; Schlessinger"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Cell"
                        "fecha" => "1990"
                        "volumen" => "61"
                        "paginaInicial" => "203"
                        "paginaFinal" => "212"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/2158859"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            12 => array:3 [
              "identificador" => "bib13"
              "etiqueta" => "13&#46;"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "The PUC plasmids&#44; and M13 mp7 derived system for insertions mutagenesis and sequencing with synlhetic universal primers"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "J&#46; Vieira"
                            1 => "J&#46; Messing"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "Gene"
                        "fecha" => "1982"
                        "volumen" => "19"
                        "paginaInicial" => "259"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/6295879"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            13 => array:3 [
              "identificador" => "bib14"
              "etiqueta" => "14&#46;"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Allelotype of colorectal carcinomas"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:7 [
                            0 => "B&#46; Vogelstein"
                            1 => "E&#46;E&#46; Fearon"
                            2 => "S&#46;E&#46; Kern"
                            3 => "S&#46;R&#46; Hamilton"
                            4 => "A&#46;C&#46; Preisinoer"
                            5 => "Y&#46; Nakamura"
                            6 => "R&#46; White"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Science"
                        "fecha" => "1989"
                        "volumen" => "244"
                        "paginaInicial" => "207"
                        "paginaFinal" => "210"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/2565047"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            14 => array:3 [
              "identificador" => "bib15"
              "etiqueta" => "15&#46;"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Ligand-induced transformation by a noninternalizing epidermal growth factor receptor"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
                            0 => "A&#46; Wells"
                            1 => "J&#46;B&#46; Welsh"
                            2 => "C&#46;S&#46; Lazar"
                            3 => "H&#46;S&#46; Wiley"
                            4 => "G&#46;N&#46; Gill"
                            5 => "M&#46;G&#46; Rosenfedl"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Science"
                        "fecha" => "1990"
                        "volumen" => "247"
                        "paginaInicial" => "962"
                        "paginaFinal" => "964"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/2305263"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            15 => array:3 [
              "identificador" => "bib16"
              "etiqueta" => "16&#46;"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Human epidermal growth factor receptor cDNA is homologous to a variety of RNAa overproduced in A431 carcinoma cells"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:9 [
                            0 => "Y&#46; Xu"
                            1 => "S&#46; Ishil"
                            2 => "A&#46;J&#46;L&#46; Clark"
                            3 => "M&#46; Sulllvan"
                            4 => "R&#46;K&#46; Wilson"
                            5 => "D&#46;P&#46; Ma"
                            6 => "B&#46;A&#46; Roe"
                            7 => "G&#46;T&#46; Merllno"
                            8 => "I&#46; Pastan"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Nature"
                        "fecha" => "1984"
                        "volumen" => "309"
                        "paginaInicial" => "806"
                        "paginaFinal" => "810"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/6330563"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
          ]
        ]
      ]
    ]
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Vol. 3. Núm. 1.
Páginas 13-19 (enero 1992)
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Vol. 3. Núm. 1.
Páginas 13-19 (enero 1992)
A very large proportion of human brain tumours show DNA alterations at the EGF-R gene
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A. Del Arco, J. García, M. Izquierdo
Departamento de Bioquímica, Biología Molecular. Universidad Autónoma de Madrid
J.M. Izquierdo*, M.G. Blázquez**
* Servicio de Neurocirugía. Hospital Valdecilla (Santander)
** Servicio de Neurocirugía. Hospital La Paz (Madrid)
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Información del artículo
Abstract

DNA alterations occurs frequently in sorne types of neoplasia such as retinoblastoma, and lung, breast and colorectal carcinoma, We have found that loses of restriction fragments at the epidermal growth factor receptor gene (EGEF-R) are present in as much as 82% of human brain tumours. Amplifications of fragments are present at a much lower proportion reaching only 10% of the samples examined. Two fragments of 9kb and 3.6kb are preferentially lost during tumorigenesis and the latter only is not present in 79% of the cases in which normal and tumoral samples from the same individual were compared. In all but one sample examined in which alterations are present, the 3.6kb fragment was heterozygotic in normal tissues and was not present in the tumour. Normal tissue surrounding the tumour was analyzed in a few examples and already showed a certain degree of alteration but normally not as much as the tumour itself. This will suggest that an accumulation ofseveral DNA aberrations might be responsible for determining the tumour biological propertieso Restriction fragment length polymorphism (RFLP), irrelevant to tumorigenesis, was found in about 22% of the cases.

Key words:
Brain tumors
Epidermal Grow Factor
EGF-R gene
DNA alterations
Resumen

Alteraciones en las cadenas de DNA han sido detectadas en algunos tipos tumorales, como retinoblastomas y carcinomas de pulmón, mama y colon. Por nuestra parte, hemos observado que en el 82% de los tumores cerebrales examinados existen pérdidas de fragmentos de DNA en el gen del receptor del factor de crecimiento epidérmico, así como amplificación de otros fragmentos en el 10% de los tumores. Los fragmentos que más frecuentemente faltan son dos, uno de 9kb (kilobase) y otro de 3,6. Este último no se encontró en el 79% de los casos en los que existía en el tejido normal del mismo paciente. En todos los casos excepto en uno, este fragmento era heterozigoto en el tejido normal y faltaba en el tumoral. El tejido aparentemente normal que rodea al tumor fue analizado en algunos casos, observándose en él algunas anomalías, aunque no tantas como en el propio tumor. Esto sugiere que probablemente sean necesarias varias alteraciones del DNA para que aparezcan las propiedades tumorales. Polimorfismo en la longitud de los fragmentos de restricción fue observado en el 22% de los casos.

Palabras clave:
Tumores cerebrales
Factor de crecimiento epidérmico
Gen receptor del EGF
Alteraciones del DNA

Artículo

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