Objectives: The cerebral representation of laughter is unclear. Prior studies had elicited laughter during electrical stimulation of the supplementary motor area and the cingulum with depth electrodes.

Methods: The present video reports a right superior frontal gyrus astrocytoma resection with awake intraoperative electrical stimulation mapping.

Results: The subcortical stimulation at the cingulate fasciculus elicited patient smile and laughter without mirth or merriment.

Conclusions: The present finding supports the role of the cingulate fasciculus in the motor aspect of laughter.